QuantiFluor™-P Fluorometer

Compact, easy to use, and cost-effective single tube fluorometer for Life Science applications.

Catalog Numbers:

E6100 QuantiFluor-P with Green/Blue Channels

E6105 QuantiFluor-P with UV/Blue Channels

Overview

The QuantiFluorâ„¢-P is a lightweight, handheld instrument configured for many of the fluorescent probes commonly used in nucleic acid and protein quantitation. While small in size, performance is not compromised. The QuantiFluorâ„¢-P has two dedicated channels that offer the ability to quickly analyze a sample for two different fluorescent signals.

Battery Powered

The QuantiFluor™-P is powered by four size AAA batteries.

Easy to Use

No special training is needed so you can focus on your data, not on how to use the software.

Inexpensive and Compact

The small, lightweight QuantiFluor™-P Fluorometer can be stored in a drawer or on a shelf. With its low price, you can buy several to easily manage the demands of a busy lab.

Dedicated Optical Channels

Two optical channels allow you to measure two different fluorophores in the same assay.

Sensitive

Four logs of dynamic range.

Internal Datalogging

An internal datalogging package with software that interfaces with Microsoft Excel can log up to 1,000 data points.

Minicell Adapter

The optional Minicell Adapter allows you to measure sample in low volumes between 75 - 250 μl.

Fluorescence Applications

Cat #E6105 UV/Blue Channel

UV Channel (Ex 365 nm, Em 440 - 470 nm)
•  Hoechst dye 33258 for DNA Quantitation
•  4-methyl-umbelliferone
Blue Channel (Ex 460 nm, Em 515 - 575 nm)
•  DNA/RNA Quantitation
•  eGFP
•  Fluorescein

Cat #E6100 Green/Blue Channel

Green Channel (Ex 525 nm, Em >570 nm)
•  Nucleotide or Protein Labeling
•  Enzyme Activity
•  Rhodamine
•  Cy3
Blue Channel (Ex 460 nm, Em 515 - 575 nm)
•  DNA/RNA Quantitation
•  eGFP
•  Fluorescein

Instrument Specifications

Detection Modes: Fluorescence
Light Source: Wavelength-matched LED
Detector: Silicon photodiode
Wavelength Selection: 2 modes
UV/Blue QuantiFluor-P Wavelengths:
UV (Ex 365, Em 440 - 470 nm),
Blue (Ex 460 nm, Em 515 - 575 nm)
Green/Blue QuantiFluor-P Wavelengths:
Green (Ex 525 nm, Em >570 nm),
Blue (Ex 460 nm, Em 515 - 575 nm)
1 ng/ml dsDNA with DNA Quantitation Dye, 1 ng/ml RNA using RNA Quantitation Dye, 10 ng/ml dsDNA using Hoechst Dye 33258, 100 ng/ml protein using Protein Quantitation Dye
Linear Dynamic Range: 4 logs, assay dependent
Read Out: Direct concentration
Calibration: Single-point calibration
Discrete Sample Average: Sample readings averaged over 5 seconds to improve accuracy
Read Type: Discrete or Continuous
Sample Format: 10 x 10 mm cuvette or 75 - 250 µl minicell vial (with optional adapter)
User Interface: Menu-driven keypad and LCD display
Data Output: ASCII format through a 9-pin
RS-232 serial cable at 9600 baud rate (optional). Optional thermal printer available.
External PC Requirements (optional): Windows XP SP2 or later
Computer Interface: RS-232 port
Power: 4 AAA alkaline batteries
Alarms: Low battery and high blank
Auto Shutoff: After 3 minutes of inactive use
Dimensions: 7.25” D x 3.5” W x 1.75” H (18.4 cm D x 8.9 cm W x 4.45 cm H)
Weight: 14 oz (0.4 kg)
Operating Temperature: 59 - 86 ºF (15 - 30 ºC)
Warranty: One year
Approvals: CE

For research use only. Not for use in diagnostic procedures. QuantiFluor is a trademark of Promega Corporation. All other trademarks are the sole property of their respective owners.