QuantiFluor™-ST Fluorometer

Compact, easy to use, and cost-effective single tube fluorometer for Life Science applications.

Catalog Number:

E6090 QuantiFluor-ST Fluorometer with UV/Blue Channels

Overview

The QuantiFluorâ„¢-ST is an affordable, sensitive fluorometer designed for quick, easy, and accurate fluorescence measurements. The QuantiFluorâ„¢-ST provides high sensitivity for fluorescence measurements and is so easy to use that you can have results within minutes. Single-point calibration saves time and the dual-channel design allows you to switch between two assays with the touch of a button.

Universal Power

The QuantiFluorâ„¢-ST is powered using a universal power adapter (included).

Small and Sensibly Priced

The QuantiFluorâ„¢-ST is smaller than a standard sized book, saving you valuable bench space. It is sensibly priced to meet your need for accurate fluorescence measurements within a limited budget.

Simple

The QuantiFluorâ„¢-ST is designed for fast, accurate fluorescence measurements. It is so easy to use that you can have results within minutes without training.

Dual Channel

Dual UV and Blue Channels allow you to switch between two assays by pressing one button.

Minicell Adapter

The optional Minicell Adapter with two focusing lenses allows you to measure samples in low assay volumes without losing sensitivity. When used with this Minicell Adapter, the QuantiFluorâ„¢-ST can detect 50 pg of dsDNA in a 50 μL assay volume.

Calibration

Single-point calibration improves accuracy while saving time.

Internal Datalogging

An internal datalogging package with software that interfaces with Microsoft Excel can log up to 1,000 data points.

Fluorescence Applications

UV Channel
•  Hoechst dye 33258 for DNA Quantitation
•  4-methyl-umbelliferone
Blue Channel
•  DNA/RNA Quantitation
•  eGFP
•  Fluorescein

Instrument Specifications

Available Detection Modes: Fluorescence
Light Source: Wavelength-matched LED
Detector: Silicon photodiode
Wavelength Selection: 2 modes
Wavelengths:
UV (Ex 365 nm, Em 440 - 470 nm)
Blue (Ex 460 nm, Em 515 - 575 nm)
Detection Limit: 1 ng/ml dsDNA with DNA Quantitation Dye, 1 ng/ml RNA using RNA Quantitation Dye, 10 ng/ml dsDNA using Hoechst Dye 33258, 100 ng/ml protein using Protein Quantitation Dye
Linear Dynamic Range: 4 logs, assay dependent
Read Out: Direct concentration
Calibration: Single-point calibration
Discrete Sample Average: Sample readings averaged over 5 seconds to improve accuracy
Read Type: Discrete or Continuous
Sample Format: 10 x 10 mm plastic cuvette or 50 - 250 µl minicell vial (with optional adapter)
User Interface: Menu-driven keypad and LCD display
Data Output: ASCII format through a 9-pin RS-232 serial cable at 9600 baud rate (optional). Optional thermal printer available.
External PC Requirements (optional): Windows XP SP2 or later
Computer Interface: RS-232 port
Power: Instrument: 9V DC, 0.3A, AC Adapter: 100 - 240V AC, 50/60Hz, 0.25A
Alarms: High blank
Dimensions: 7.25” D x 5.5” W x 2.7” H (18.42 cm D x 14 cm W x 6.9 cm H)
Weight: 28.8 oz (0.67 kg)
Operating Temperature: 59 - 86 ºF (15 - 30 ºC)
Warranty: One year
Approvals: CE, UL-listed power supply

 For research use only. Not for use in diagnostic procedures. QuantiFluor is a trademark of Promega Corporation. All other trademarks are the sole property of their respective owners.