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Picofluor™ Handheld Fluorometer

Compact, easy to use, and cost-effective single tube fluorometer for Life Science applications.

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Overview

The Picofluor™ Fluorometer is a lightweight, handheld instrument configured for many of the fluorescent probes commonly used in nucleic acid and protein quantitation. While small in size, performance is not compromised. The Picofluor™ has two dedicated channels that offer the ability to quickly analyze a sample for two different fluorescent signals.

Easy to Use

No special training is needed so you can focus on your data, not on how to use the software.

Sensitive

Four logs of dynamic range.

Inexpensive and Compact

The small, lightweight Picofluor™ Fluorometer can be stored in a drawer or on a shelf. With its low price, you can buy several to easily manage the demands of a busy lab.

Dedicated Optical Channels

Two optical channels allow you to measure two different fluorophores in the same assay.

Battery Powered

The Picofluor™ is powered by four size AAA batteries.

Internal Datalogging

An internal datalogging package with software that interfaces with Microsoft Excel can log up to 1,000 data points.

P/N 8000-003 UV/Blue Channel

UV Channel
•  Hoechst dye 33258 for DNA
   Quantitation
•  4-methyl-umbelliferone
Blue Channel
•  Picogreen® for DNA
   Quantitation
•  Ribogreen® for RNA
   Quantitation
•  eGFP
•  Fluorescein

P/N 8000-004 Green/Blue Channel

Green Channel
•  Nucleotide or Protein Labeling
•  Enzyme Activity
•  Rhodamine
•  Cy3
Blue Channel
•  Picogreen® for DNA
   Quantitation
•  Ribogreen® for RNA
   Quantitation
•  eGFP
•  Fluorescein

Picofluor™ Handheld Fluorometer
Instrument Specifications

Detection Modes: Fluorescence
Light Source: Wavelength-matched LED
Detector: Silicon photodiode
Wavelength Selection: 2 modes
Wavelengths: P/N 8000-003
UV (Ex 365, Em 440 - 470 nm),
Blue (Ex 460 nm, Em 515 - 575 nm)
Wavelengths: P/N 8000-004
Green (Ex 525 nm, Em >570 nm),
Blue (Ex 460 nm, Em 515 - 575 nm)
Detection Limit: 1 ng/ml dsDNA with PicoGreen®, 1 ng/ml RNA using RiboGreen®,
10 ng/ml dsDNA using Hoechst Dye 33258,
100 ng/ml protein using NanoOrange®
Linear Dynamic Range: 4 logs, assay dependent
Read Out: Direct concentration
Calibration: Single-point calibration
Discrete Sample Average: Sample readings averaged over 5 seconds to improve accuracy
Read Type: Single
Sample Format: 10 x 10 mm cuvette or
75 - 250 µl minicell vial (optional)
User Interface: Menu-driven keypad and LCD display
Data Output: ASCII format through a 9-pin
RS-232 serial cable at 9600 baud rate (optional)
External PC Requirements (optional): Windows 95 or later
Computer Interface: RS-232 port
Power: 4 x AAA batteries
Alarms: Low battery, circuit failure, and high blank
Auto Shutoff: After 90 seconds of inactive use
Dimensions: 7.25” D x 3.5” W x 1.75” H
(18.4 cm D x 8.9 cm W x 4.45 cm H)
Weight: 14 oz (0.4 kg)
Operating Temperature: 41 - 104 ºF (5 - 40 ºC)
Warranty: One year
Approvals: CE, meets IP67 Standard

For research use only. Not for use in diagnostic procedures. Picofluor is a trademark of Turner BioSystems, Inc. All other trademarks are the sole property of their respective owners.

 

 


 
Promega Corporation