GloMax® 20/20 Single Tube Luminometer

An ultra-sensitive, versatile, and affordable single tube luminometer for Life Science research.

E5311 Instrument with No Injectors

E5321 Instrument with Single Injector

E5331 Instrument with Dual Injectors

Optimized for these Promega Reagents

For easy ordering, click on the Catalog Number links below to open a new browser window containing the Promega on-line catalog.

Dual-Glo™ Luciferase Assay System, E2920

Dual-Luciferase® Reporter Assay System , E1910

Chroma-Glo™ Luciferase Assay System ,  E4910

Steady-Glo® Luciferase Assay System, E2510

Bright-Glo™ Luciferase Assay System, E2610

Glo Lysis Buffer,  E2661

Luciferase Assay System,  E1500

Renilla Luciferase Assay System,  E2810

Renilla-Gloâ„¢ Luciferase Assay System, E2710

Overview

Superior Performance

The GloMax® 20/20 Luminometer provides exceptional sensitivity for all luminescent assays. Proprietary circuitry and an advanced photon-counting photomultiplier tube (PMT) produce unmatched signal-to-noise ratios. The GloMax® 20/20 has a detection limit of 1 x 10^-21 moles of luciferase, making it one of the most sensitive tube luminometers available.

With greater than 8 logs of linear dynamic range, the GloMax® 20/20 Luminometer can measure both dim and bright samples without dilution. The extended dynamic range spans the full range of virtually all chemiluminescent and bioluminescent assays, eliminating the need to dilute samples or manage detector-driven gain changes.

Ease of Use

The GloMax® 20/20 is designed to be put into use straight from the box without the need to read a manual or obtain special training. To achieve this plug-and-play usability, the GloMax® 20/20 combines a color touch screen with an intuitive user interface. The interface makes running samples and viewing data fast and simple while also maintaining the flexibility needed for advanced or custom protocols.

Versatility

The GloMax® 20/20 is a versatile addition to any lab. The optional single or dual auto-injectors allow measurement of either flash or glow-type luminescence, while the flexible sample compartment accommodates 1.5 ml microcentrifuge tubes, 35 mm petri dishes, and an optional 12 mm x 50 mm test tube adapter. It is also possible to measure the light output from both liquid and solid samples. In addition, the bottom reading design means there are no minimum volume requirements. For added functionality, two fluorescent modules are available, expanding the GloMax® 20/20 functionality to common fluorescence applications.

Flexible Sample Compartment

The GloMax®-20/20 accommodates 1.5 ml microcentrifuge tubes, 35 mm petri dishes, and 12 mm x 50 mm test tubes (with optional adapter). It is also possible to measure the light output from both liquid and solid samples.

Lid Start

Adjust settings to start measurement immediately after the lid is closed.

Touch Screen

The touch screen allows simple navigation between screens for setting parameters, storing protocols, and starting runs.

Data Handling

The GloMax®-20/20 stores up to 18 protocols and displays data from the last 20 measurements on the LCD screen. Spreadsheet Interface Software to connect to a PC, and an optional thermal printer are also available for easy data exporting.

Intuitive User Interface

Luminescence

Choose from pre-loaded Promega protocols, the default protocol, or create your own custom protocol. Use the Instrument Control screen to edit parameters, then press MEASURE LUMINESCENCE.

Fluorescence

Snap in one of two Fluorescence Modules, power cycle the instrument, and the GloMax®-20/20 automatically adjusts screens and options.

Injector Control

Control injectors from the touch screen. Both PRIME and FLUSH commands are integrated into the software.

Single or Dual Auto Injectors (Optional)

Injectors dispense fluids from 25 - 300 μl and enable detection of flash-based luminescence or dual-reporter assays. Completely visible plumbing allows inspection of tubing and tips during flushing and priming.

Luminescence Applications

Luminescence

To achieve the GloMax®-20/20's high sensitivity, the luminescence channel is positioned directly below the sample well. These conditions maximize light capture. In addition, a low-noise photomultiplier tube ensures that collected light is not compromised. The GloMax®-20/20 has greater than 8 logs of dynamic range. This dynamic range is more than adequate to cover common luminescence applications, thus reducing the need to dilute samples.

Luminescence Light Standard

The GloMax®-20/20 Luminometer Light Standard provides an external control to verify performance. Some labs require this additional verification procedure. Reading the light standard before taking measurements is a quick and easy way to ensure quality control of reproducibility, sensitivity, and linearity of measurements.

Fluorescence Applications (optional)

Fluorescence

Optional Fluorescence Modules add functionality to the GloMax®-20/20. The modules are designed to measure common fluorescence applications using excitation wavelengths in the near UV or Blue light range. The Fluorescence Modules provide sensitive measurements of DNA, RNA, or protein quantitation dyes.

Instrument Specifications

Available Detection Modes: Luminescence, Fluorescence (optional)
Detector: Photomultiplier tube (PMT)
Spectral Range: 350 - 650 nm
Peak Wavelength: 420 nm
Detection Limit: 3 x 10^-18 moles ATP or 1 x 10^-21 moles Luciferase
Linear Dynamic Range: >8 logs
Read Type: Glow, Flash, Kinetic, Repeat
Sample Format: 1.5 ml microcentrifuge tubes, 35 mm petri dishes, 12 mm x 50 mm test tubes (optional), minicell vials (optional, Fluorescence)
Sample Adapter: Holds 1.5 ml microcentrifuge tubes, optional 12 mm x 50 mm test tube holder available
User Interface: Touch screen navigation and operation
Data Output: Data displayed on screen or connect to PC (not included) via serial cable to download. Optional thermal printer available.
External PC Requirements (optional): Windows XP SP2 or higher
Computer Interface: 100% ASCII format through a 9-pin RS-232 serial cable at 9600 baud rate
Power: 12 V 0.84 A Max
Auto Shutoff: Hibernates after 15 minutes of inactivity
Dimensions: 12.92” D x 10.44” W x 8.42” H (32.82 cm D x 26.52 cm W x 21.39 cm H)
Weight: 8.4 lbs (3.81 kg)
Operating Temperature: 60 - 105 ºF (15 - 40 ºC)
Warranty: One year
Approvals: CE

Single Auto Injector System (Optional)

Number of Injectors: One injector
Dispense Volume Range: Selectable between 25 - 300 µl in 5 µl increments

Dual Auto Injector System (Optional)

Number of Injectors: Two injectors
Dispense Volume Range: Selectable between 25 - 300 µl in 5 µl increments

Fluorescence Specifications (User Installable)

Light Source: Wavelength-matched LED
Detector: PMT
UV Fluorescence Module
• Wavelengths: Ex 365 nm, Em 440 - 470 nm
• Detection Limit: 10 ng/ml dsDNA using Hoechst Dye 33258
Blue Fluorescence Module
• Wavelengths: Ex 460 nm, Em 515 - 575 nm
• Detection Limit: 450 pg/ml dsDNA with DNA Quantitation Dye
Linear Dynamic Range: 5 logs, assay dependent
Sample Adapter: 100 - 200 µl minicell vial
Read Out: Fluorescence Standard Units, Direct Concentration
Calibration: Single-point calibration
Discrete Sample Average: Sample readings averaged over 5 seconds to improve accuracy

For research use only. Not for use in diagnostic procedures. GloMax is a registered trademark of Promega Corporation. All other trademarks are the sole property of their respective owners.