Multimode Reader - GloMax-Multi+ Microplate Multimode Reader for Life Sciences. Affordable, easy to use, with touchscreen interface. From Promega Corporation. Turner BioSystems and Promega: Sensitive, affordable, and easy to use luminometers, fluorometers, and multimode readers for life sciences luminescence, fluorescence, and absorbance applications. Maxwell-16 for Automated DNA Purification.
The GloMax®-Multi+ replaces the Turner BioSystems Modulus II Microplate Multimode Reader. Same instrument... new look!


GloMax-Multi+ Microplate Multimode Reader


Catalog Numbers:

E8032 Instrument with Shaking
E9032 Instrument with Heating and Shaking
E8041 Luminescence Module (Factory Installed)
E8051 Fluorescence Module
E8061 Visible Absorbance Module
E9061 UV-Visible Absorbance Module
E7071 Single Injector System
E7081 Dual Injector System

For easy ordering, click on the Catalog Number links below to open a new browser window containing the Promega on-line catalog.

Dual-Reporter Assays

Dual-Glo™ Luciferase Assay, E2920 (Luminescence Module, Dual Injector System)

Dual-Luciferase®Reporter Assay System E1910 (Luminescence Module, Dual Injector System)

Firefly Luciferase Reporter Assays

Luciferase Assay System E1500 (Luminescence Module, Single Injector System)

Bright-Glo™ Luciferase Assay System,  E2610 (Luminescence Module)

Steady-Glo® Luciferase Assay System, E2510 (Luminescence Module)

Renilla Luciferase Reporter Assays

EnduRen™ Live Cell Substrate, E6481 (Luminescence Module)

ViviRen™ Live Cell Substrate,  E6491 (Luminescence Module)

Renilla-Gloâ„¢ Luciferase Assay System E2710

Cell Viability Assays

CellTiter-Glo® Luminescent Cell Viability Assay, G7570 (Luminescence Module)

CellTiter-Blue®Cell Viability Assay , G8080 (Fluorescence Module, Green Optical Kit)

CellTiter-Fluor™ Cell  Viability Assay,  G6080 (Fluorescence Module, AFC Optical Kit)

CellTiter 96® AQueous One Solution  Cell Proliferation Assay, G3850 (Absorbance Module,  490nm Filter)

CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay, G5421 (Absorbance Module,  490nm Filter)

BacTiter-Glo™ Microbial Cell Viability Assay, G8230 (Luminescence Module)

MultiTox-Fluor Multiplex Cytotoxicity Assay, G9200 (Fluorescence Module, AFC Optical Kit, Blue Optical Kit)

MultiTox-Glo Multiplex Cytotoxicity Assay* G9270  (Fluorescence Module,  AFC Optical Kit, Luminescence Module)

CytoTox-ONE™ Homogeneous Membrane Integrity Assay, G7891  (Fluorescence Module, Green Optical Kit)

CytoTox-Fluor™ Cytotoxicity Assay,  G9260 ( Fluorescence Module, Blue Optical Kit)

CytoTox 96® Cytotoxicity Assay,  G1780 (Absorbance Module, 490nm Filter)

CytoTox-Glo™ Cytotoxicity Assay,  G9290 (Luminescence Module)

Luminescent Apoptosis Assays

Caspase-Glo® 3/7 Assay, G8091 (Luminescence Module)

Caspase-Glo® 8 Assay, G8201 (Luminescence Module)

Caspase-Glo® 9 Assay, G8211 (Luminescence Module)

Apo-ONE®Homogeneous Caspase-3/7 Assay, G7792 (Fluorescence Module, Blue Optical Kit)

Cell Signaling

cAMP-Glo™ Assay V1501 (Luminescence Module)

PDE-Glo™ Phosphodiesterase Assay V1361 (Luminescence Module)

CYP450 Screening Systems

P450-Glo™ CYP1A2 Screening System V9770 Luminescence Module

P450-Glo™ CYP2C9 Screening System V9790 Luminescence Module

P450-Glo™ CYP3A4 Screening System V9800 Luminescence Module

P450-Glo™ CYP2C19 Screening System V9880 Luminescence Module

P450-Glo™ CYP2D6 Screening System V9890 Luminescence Module

ADME Assays

p450-Glo™ CYP3A4 Screening System (Luciferin - PPXE) DMSO-Tolerant Assay, V9910 (Luminescence Module)

MAO-Glo™ Assay, V1401 (Luminescence Module)

GloMax®-Multi+ Microplate
Multimode Reader with Instinct
â„¢

A modular, easy to use and cost effective multimode reader for Luminescence, Fluorescence, and Absorbance

OVERVIEW

The GloMax®-Multi+ Microplate Multimode Reader is designed for today’s life science laboratory. In addition to giving performance equivalent to that of single-mode instruments, the GloMax®-Multi+ blends user-friendly operation with easy data handling and flexible purchasing options. The result of this design is an instrument with superior performance that is easy to use, is affordable, and can be customized to your laboratory’s needs.

PERFORMANCE

The GloMax®-Multi+ Microplate Multimode Reader is designed for today's life science laboratory. In addition to giving performance equivalent to that of single-mode instruments, the GloMax®-Multi+ blends user-friendly operation with easy data handling and flexible purchasing options. The result of this design is an instrument with superior performance that is easy to use, is affordable, and can be customized to your laboratory's needs.

EASE OF USE

The GloMax®-Multi+ Microplate Multimode Reader is designed to be put into use straight from the box, without the need for special training. The built-in computer eliminates the connectivity hassles, cost, and space requirements of running an external computer. Assay set-up on the GloMax®-Multi+ is effortless with several flexible set-up options to choose from.

AFFORDABLE MODULARITY

The GloMax®-Multi+ Microplate Multimode Reader is a modular instrument that easily fits into most budgets. Purchase the technology or modes that you need now and add onto the system later as your needs expand. For example, the GloMax®-Multi+ can be purchased as a luminometer, then fluorescence and/or absorbance modules can be purchased and added later.
Installations take less than ten minutes and can be done right in your lab using just the Allen wrench provided with the module. In addition, after installing a new module, you will not need to download and install new software. The GloMax®-Multi+ instantly detects the newly installed module(s) and will automatically adjust screens, protocols, and options.

GloMax-Multi+ Microplate Multimode Reader

DATA HANDLING

To transfer data to a PC, simply copy the data from the GloMax®-Multi+ then move the USB stick (included) to your computer. Analyze your data when and where you find it most convenient.

TEMPERATURE CONTROL (OPTIONAL)

The GloMax®-Multi+ may be purchased with an optional heater, allowing precise temperature control from 2º above ambient temperature to 45º C.

SHAKING

The GloMax ®-Multi+ is configured with a factory-installed shaker that allows either linear or orbital shaking.

MICROPLATE FORMAT

Accepts 6, 12, 24, 48, 96, and 384-well plates conforming to the SBS plate standard.

COMPUTER INTERFACE

For those labs that would prefer to operate the GloMax®-Multi+ through an external PC, the PC Software contains all of the same ease-of-use features that are available via the instrument's built-in touch screen.

INJECTORS

Single or dual auto injectors are available for flash-based luminescence applications or dual-reporter assays. Each injector system has a volume range of 25 - 200 μl in 5μl increments for 6- to 96-well plate formats. Injectors are controlled using the touch screen and fluidics wizard. PRIME and FLUSH commands provide easy maintenance and a REVERSE PURGE command saves valuable reagents.

COLOR TOUCH SCREEN

The GloMax®-Multi+ combines a 6.6" color touch screen display with an onboard Windows-based computer.

INTUITIVE USER INTERFACE

Setting up a run and retrieving data are fast and simple while maintaining the flexibility needed for advanced or custom protocols.

PROTOCOL COMPOSER

Complex protocols can easily be developed by combining multiple technologies into one experiment. Save time and reagents by testing for both luminescence and fluorescence, or fluorescence and absorbance, in one run.

INSTINCT SOFTWARE

With Instinctâ„¢ software, included with GloMax Multi+ instruments, you can perform data analysis right on the touch screen. With a few simple button selections, label your data, choose an analysis model and see the analyzed results after the data is collected.

TOUCH SCREEN SELECTION

The Windows-based computer built into the GloMax-Multi+ Microplate Multimode Reader offers dynamic user-interface capabilities. Simply choose from three options on the instrument touch screen to start setting up a run.

GloMax-Multi+ with Instinct - Home Screen

CREATE A NEW PROTOCOL

Use the NEW PROTOCOL wizard to create a customized protocol. The wizard guides you step-by-step through choosing read parameters, injection methods, and plate wells to read, and in saving your protocol for future use.

GloMax-Multi+ with Instinct Create a New Protocol

CHOOSE A PRELOADED PROTOCOL

Choose SELECT PROTOCOL to access to the most popular assays from common reagent suppliers, or instantly access previously saved user-customized protocols.

GloMax-Multi+ with Instinct - Choose a Preloaded Protocol

TOTAL INSTRUMENT CONTROL

From the INSTRUMENT CONTROL screen, simply touch the parameter to change or customize all settings necessary to obtain the perfect read for your application. Once your parameters are set, you can save your settings for future use.

GloMax-Multi+ with Instinct - Total Instrument Control

INSTINCTâ„¢ SOFTWARE PLATE MAP

Use the advanced plate map features to select a curve fit model, create sample groups, and label wells.

GloMax-Multi+ with Instinct - Plate Map

INSTINCT SOFTWARE RESULTS

Results screens display raw data, calculated concentrations and graphs for instant review of your data.

GloMax-Multi+ with Instinct - Software Results

PROTOCOL COMPOSER

From the INSTRUMENT CONTROL screen, move a parameter into the Protocol Composer list to create multi-parameter protocols. Complex protocols can easily be developed by combining multiple technologies into one experiment. Save time and reagents by testing for both luminescence and fluorescence, or fluorescence and absorbance, in one run.

DETECTION MODES

Luminescence Module
(Factory Installed)

>8 Logs Dynamic Range
Dual-masking System Reduces Crosstalk
3 x 10-21 Moles Luciferase Detection or 1 x 10-18 moles ATP

To achieve the sensitivity of a dedicated luminometer, the luminescence channel is separated from other measurement technologies and positioned directly above the sample well. These conditions maximize light capture for the best possible sensitivity. In addition, a low-noise photomultiplier tube ensures that collected light is not compromised in any way. This design makes the GloMax-Multi+ Microplate Multimode Reader between 10 to 1000 times more sensitive than competing multimode luminometers. In addition to high sensitivity, the GloMax®-Multi+ boasts greater than 8 logs of dynamic range, eliminating the need to dilute samples or manage detector-driven gain changes. The photomultiplier tube automatically adjusts for the optimum reading of bright or dim samples. This means that the GloMax®-Multi+ is capable of achieving a reading range 2 - 3 logs more than that of competing multimode luminometers.

Luminescence Injector/Application Chart

Recommended Injectors

Applications

2

Dual Reporter Assays

0

Steady-Glo® Luciferase Assays

1

Flash Glow Luciferase Assays

1

Cell Viability/ATP Assays

1

Kinetics Assays

1

Calcium (Aequorin)

Fluorescence Module
(User Installable)

Epifluorescent Detection (top reading)
Easy Optical Kit Switching
Wavelength-matched LEDs Ensure High Sensitivity

To achieve high performance, the Fluorescence Module uses powerful light-emitting diodes (LEDs) as excitation sources. LEDs have very specific light-output profiles that closely match the excitation profiles of commonly used fluorescent molecules. LED usage increases sensitivity by fully exciting the fluorophore and reducing non-specific light leakage, a common problem when using broad-spectrum light sources. Four standard optical kits are available to measure the most popular fluorophores. In addition, custom optical kits can be readily made for non-standard applications. Optical kits can be easily exchanged in seconds and built-in software ensures that the installed optical kit matches the selected protocol. Designed as a user-installable module, you can either buy the Fluorescence Module with your GloMax®-Multi+ instrument or add it to your system later when you are ready to run fluorescence experiments.

Fluorescence Module Optical Kit Application Chart

Fluorescence
Optical Kit

Typical Applications

UV Optical Kit
Ex: 365 nm
Em: 410 - 460 nm

DNA Quantification (Hoechst dye), Enzyme Activity (4-methyl-umbelliferone)

Blue Optical Kit
Ex: 490 nm
Em: 510 - 570 nm

DNA Quantitation, RNA Quantitation, Protein Labeling (Fluorescein), Protein Quantitation, Gene Expression (EGFP, rAcGFP)

Green Optical Kit
Ex: 525 nm
Em: 580 - 640 nm

Nucleotide or Protein Labeling (Rhodamine, Cy3), Enzyme Activity (Rhodamine)

Red Optical Kit
Ex: 625 nm
Em: 660 - 720 nm

Nucleotide or Protein  Labeling (Cy5),
RNA Quantitation

Absorbance Module
(User Installable)

UV-Visible or Visible Modules
Flexible Filter System
Reads Single or Dual Wavelengths

The GloMax-Multi+ Microplate Multimode Reader provides you with two different options for making photometric measurements. A Visible Absorbance Module (400 - 800 nm) or a UV-Visible Absorbance Module (200 - 1100 nm). Both modules are user installable, so there is no downtime or need for a service call.

Both the Visible and UV-Visible Absorbance Modules come factory-installed with four common filters: 450, 560, 600, and 750 nm wavelengths. The UV-Visible module also comes with a 260 and 280 nm filter. In addition, you can customize either module by inserting a filter of your choice into either of two removable filter paddles.

Absorbance Module Filter Application Chart

Wavelength

Applications

260

DNA and RNA Quantification

280

Protein Quantitation

450

ELISA, QuantiCleave™ Protease Assay

560

BCA™ Protein Assays

600

Bradford Protein Assays, Coomassie Blue Protein Assays, PeroXOquant™ Quantitative Peroxide Assay

750

Lowry Protein Assay

SPECIFICATIONS

GLOMAX®-MULTI+

Available Detection Modes

Luminescence, Fluorescence, Visible Absorbance, UV-Visible Absorbance

Read Type

Glow, Flash, Kinetic, Repeat

Sample Format*

 6, 12, 24, 48, 96, 384-well plates

Shaking**

Four speeds (150, 300, 500 rpm, Variable) linear and orbital modes

Temperature Control**

2 ºC above ambient temperature to 45 ºC +/- 0.75 ºC. Order Instrument Cat #E9032

User Interface

Built-in PC, touch screen navigation and operation

Data Output***

PC compatible ASCII text file format exported to USB flash drive, or connect to PC (not included) through USB or Serial Port

External PC Requirements (optional)

Windows XP or Vista

Power

100 - 240 VAC, 50 - 60 Hz

Auto Shutoff

Touch screen hibernates after 15 min of inactivity

Dimensions

21” D x 17.3” W x 12.83” H  (53 cm D x 44 cm W x 32.6 cm H)

Weight

~35 lbs (~16 kg)

Operating Temperature

 60 - 85 ºF (15 - 30 ºC)

Warranty

One year parts and labor

Approvals

CE

LUMINESCENCE

Detector

Head-on photomultiplier tube (PMT) for photon counting

Spectral Range

350 - 650 nm

Peak Wavelength

420 nm

Detection Limit

3 x 10-21 moles of luciferase or 1 x 10-18 moles of ATP

Linear Dynamic Range

 >8 logs

Crosstalk

5 x 10-5

SINGLE INJECTOR SYSTEM

Number of Injectors

One injector

Dispense Volume Range

Selectable between 25 - 200 μl in 5 μl increments for 6 to 96-well plate formats.

Waste Tray Volume

~50 ml

DUAL INJECTOR SYSTEM

Number of Injectors

Two injectors

Dispense Volume Range

Selectable between 25 - 200 μl in 5 μl increments for 6 to 96-well plate formats.

Waste Tray Volume

~50 ml

FLUORESCENCE

Light Source

Wavelength-matched LED

Detector

PIN-photodiode

Read Position

Top reading

Wavelength Selection

Snap-in Fluorescence Optical Kits

Wavelengths

UV (Ex 365 nm: Em 410 - 460 nm), Blue (Ex 490 nm: Em 510 - 570 nm), Green (Ex 525 nm: Em 580 - 640 nm), Red (Ex 625 nm: Em 660 - 720 nm)

Detection Limit

0.5 fmol/200 μl or 1 ppt of fluorescein in 96-well plate, 30 pg/well dsDNA with DNA Quantification Dye

Linear Dynamic Range

6 logs, assay dependent

Calibration**

Multipoint Calibration

Read Out

Relative Fluorescence units, Direct Concentration

Discrete Sample Average

Sample readings averaged over 0.5 seconds to improve accuracy

VISIBLE ABSORBANCE

Light Source

LED

Detector

Large-area photodiode

Spectral Range

400 - 800 nm

Wavelengths for Installed Filters

450, 560, 600, 750 nm, 2 slots for customization

Photometric Measuring Range

0 - 5.0 OD

Linear Dynamic Range

0 - 4.0 OD, assay dependent

OD Accuracy

0.01 OD ± 3% at ≤ 2.5 OD

OD Precision

0.01 OD ± 1%

Stray Light

0.002% @ 560 nm in clear bottom, black wall plate

UV-VISIBLE ABSORBANCE

Light Source

Xenon lamp

Detector

Photodiode

Spectral Range

200 - 1100 nm

Wavelengths for Installed Filters

260, 280, 450, 560, 600, 750 nm (2 slots customizable)

Photometric Measuring Range

0 - 4.0 OD

Linear Dynamic Range

0 - 3.5 OD, assay dependent

OD Accuracy

0.05 OD ± 3% at ≤ 2.5 OD

OD Precision

0.002 ± 3%

Stray Light

0.001% @ 560 nm in clear bottom, black wall plate

*The GloMax®-Multi Microplate Multimode Reader uses 96-well plates only.
**Feature is not available on the GloMax®-Multi Microplate Multimode Reader.
***Data Output for the GloMax®-Multi Microplate Multimode Reader includes MAC compatible and .csv file format.

For research use only. Not for use in diagnostic procedures. GloMax is a trademark of Promega Corporation. All other trademarks are the sole property of their respective owners. For the most up-to-date specifications, visit www.promega.com.

 

 


 
Promega Corporation